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Can the presence of Biofilms be identified under routine H&E staining?

RhinologyCenters for disease control and prevention estimate that about 65% of human bacterial infectious processes involve biofilms.

Biofilm is a group of bacteria seen over surface epithelium like mucous membrane, enclosed within a matrix of extracellular

polysaccharide material. The presence of this biofilm has been implicated in the following chronic infections:

1. Chronic rhinosinusitis

2. Chronic bronchitis

3. Atrophic rhinitis

4. Lung infections following prolonged intubation

5. Urinary catheter related infections

How biofilms protect the bacteria from host defenses?

Biofilm contains multiple types of bacteria. Bacteria from biofilms are difficult to culture & hence difficult to identify. These

multiple organisms coexist with each other conferring drug resistance. Antibiotics don't penetrate biofilms in adequate

concentrations thus the organisms within are well entrenched and protected. These biofilms surround the microbes with extracellular polymeric substance which

constitute a physical non cellular barrier against host defense mechanisms. Staphylococcus and pseudomonas organisms have been implicated in chronic sinonasal disease associated biofilms.

How to identify Biofilms?

Biofilms have been identified by scanning electron microscopy.

Recently Fluorescence insitu hybridization techniques have been used to identify biofilms (FISH technique).

Christian J. Hochstim in his original work has shown that the presence of Biofilms can be identified with reasonable degree of accuracy using H & E stains.

Histologically under H & E staining, biofilms appear as clusters of basophilic bacteria and host cells entrapped in a layer of extracellular polymeric substance.

It is really worthwhile looking for the presence of biofilms in all mucosal specimen sent for biopsy.

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Een goede krabpaal moet ook stevig staan. Hij mag niet omvallen als de kat er met een stevige sprong in of uit springt. Grote krabpalen die tot het plafond reiken komen meestal met een plafond spanner, waarmee je de paal tussen de vloer en plafond kan ... [read more]
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